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It's a thermostable ligase discovered in quite a few thermophilic germs, they have to have NAD+ for a cofactor.
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The image demonstrates how ligase (yellow oval) catalyzes two DNA fragment strands. The ligase joins the two fragments of DNA to variety a longer strand of DNA by "pasting" them jointly. The mechanism of DNA ligase is usually to variety two covalent phosphodiester bonds in between 3' hydroxyl finishes of 1 nucleotide ("acceptor"), With all the five' phosphate close of Yet another ("donor").
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DNA ligase is usually a kind of enzyme that facilitates the becoming a member of of DNA strands jointly by catalyzing the development of the phosphodiester bond. It plays a job in restoring solitary-strand breaks in duplex DNA in residing organisms, but some forms (for instance DNA ligase IV) may well precisely repair service double-strand breaks (i.
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